CRY2olig-mCh-CLC
(Plasmid #60035)

• Purpose: Expresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chain
• Depositing Lab: Chandra Tucker
• Publication: Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.1038/ncomms5925.

Backbone

• Vector backbone: pmCherryN1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 6850
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRY2-mCh-Clathrin Light Chain
• Alt name: CRY2
• Alt name: Clathrin Light Chain
• Species: M. musculus (mouse), A. thaliana (mustard weed)
• Insert Size (bp): 2892
• Mutation: M354I, E490G CRY2, A9D*
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: mCherry
• 3′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*This plasmid contains clathrin light chain A, isoform a, with an A9D mutation relative to NM_001080384 (NP_001073853). This mutation, however, seems to be a natural variant, as it is present in other published sequences for mouse Clta