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Addgene

MSCV-TAP(FLAG-HA) CYLD
(Plasmid #60031)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60031 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV-N-Flag-HA-IRES-PURO
  • Backbone manufacturer
    Harper lab (Addgene Plasmid #41033)
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cylindromatosis
  • Species
    H. sapiens (human)
  • Entrez Gene
    CYLD (a.k.a. BRSS, CDMT, CYLD1, CYLDI, EAC, FTDALS8, MFT, MFT1, SBS, TEM, USPL2)
  • Promoter LTR-driven expression
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • HA (N terminal on backbone)
    • IRES Puro (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CAGCCCTCACTCCTTCTCTAGG
  • 3′ sequencing primer GCATTCCTTTGGCGAGAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-TAP(FLAG-HA) CYLD was a gift from Stephen Elledge (Addgene plasmid # 60031 ; http://n2t.net/addgene:60031 ; RRID:Addgene_60031)

  • For your References section:

    The tumor suppressor CYLD regulates entry into mitosis. Stegmeier F, Sowa ME, Nalepa G, Gygi SP, Harper JW, Elledge SJ. Proc Natl Acad Sci U S A. 2007 May 22. 104(21):8869-74. 10.1073/pnas.0703268104 PubMed 17495026
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