pTRE-Tight-FLAG-CYLD-WT
(Plasmid
#60030)
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PurposeWT CYLD under control of Tet promoter with N-terminal FLAG tag
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60030 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR
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Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 2600
- Total vector size (bp) 3000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCylindromatosis
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SpeciesH. sapiens (human)
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Insert Size (bp)400
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Entrez GeneCYLD (a.k.a. BRSS, CDMT, CYLD1, CYLDI, EAC, FTDALS8, MFT, MFT1, SBS, TEM, USPL2)
- Promoter TRE
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer TREF (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTRE-Tight-FLAG-CYLD-WT was a gift from Stephen Elledge (Addgene plasmid # 60030 ; http://n2t.net/addgene:60030 ; RRID:Addgene_60030) -
For your References section:
The tumor suppressor CYLD regulates entry into mitosis. Stegmeier F, Sowa ME, Nalepa G, Gygi SP, Harper JW, Elledge SJ. Proc Natl Acad Sci U S A. 2007 May 22. 104(21):8869-74. 10.1073/pnas.0703268104 PubMed 17495026