pTRE-Tight-FLAG-CYLD-C601A
(Plasmid #60029)

• Purpose: CYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tag
• Depositing Lab: Stephen Elledge
• Publication: Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 104(21):8869-74.

Backbone

• Vector backbone: pENTR
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 2600
• Total vector size (bp): 3000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cylindromatosis
• Species: H. sapiens (human)
• Insert Size (bp): 400
• Mutation: Cysteine 601 changed to Alanine
• Entrez Gene: CYLD (a.k.a. BRSS, CDMT, CYLD1, CYLDI, EAC, FTDALS8, MFT, MFT1, SBS, TEM, USPL2)
• Promoter: TRE
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: TREF

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry