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PurposeExpression of a TALEN protein specific for mouse ROSA26 locus.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60026 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonephRL-SV40
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Backbone manufactureraddgene
- Total vector size (bp) 6000
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Modifications to backboneinsertion of TALE DNA binding domain via Golden gate cloning containing following RVDs: NN-HD-HD-HD-NI-NN-NI-NI-NN-NI-HD-NG-HD-HD-HD-NN-HD
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Vector typeMammalian Expression, TALEN
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTALEN-ROSA26
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TALEN-mROSA26 ELD was a gift from Radislav Sedláček (Addgene plasmid # 60026 ; http://n2t.net/addgene:60026 ; RRID:Addgene_60026) -
For your References section:
Efficient gene targeting of the Rosa26 locus in mouse zygotes using TALE nucleases. Kasparek P, Krausova M, Haneckova R, Kriz V, Zbodakova O, Korinek V, Sedlacek R. FEBS Lett. 2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 10.1016/j.febslet.2014.09.014. 10.1016/j.febslet.2014.09.014 PubMed 25241166