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Addgene

TALEN-mROSA26 KKR
(Plasmid #60025)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60025 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    phRL-SV40
  • Backbone manufacturer
    addgene
  • Total vector size (bp) 6000
  • Modifications to backbone
    insertion of TALE DNA binding domain via Golden gate cloning containing following RVDs: HD-NN-NG-NN-NI-NG-HD-NG-NN-HD-NI-NI-HD-NG-HD-HD-NI-NN-NG
  • Vector type
    Mammalian Expression, TALEN

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TALEN-ROSA26
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (unknown if destroyed)
  • 3′ cloning site BsmBI (unknown if destroyed)
  • 5′ sequencing primer gaggagtcacggcggtc
  • 3′ sequencing primer catccaaggcgggtcgt
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TALEN-mROSA26 KKR was a gift from Radislav Sedláček (Addgene plasmid # 60025 ; http://n2t.net/addgene:60025 ; RRID:Addgene_60025)

  • For your References section:

    Efficient gene targeting of the Rosa26 locus in mouse zygotes using TALE nucleases. Kasparek P, Krausova M, Haneckova R, Kriz V, Zbodakova O, Korinek V, Sedlacek R. FEBS Lett. 2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 10.1016/j.febslet.2014.09.014. 10.1016/j.febslet.2014.09.014 PubMed 25241166
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