pCiPS
(Plasmid #60011)

• Purpose: Foamy virus helper plasmid expressing Pol, driven by a CMV promoter. Ampicillin resistant.
• Depositing Lab: David Russell
• Publication: Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

Backbone

• Vector backbone: pCMV beta
• Backbone manufacturer: unknown
• Backbone size w/o insert (bp): 7164
• Total vector size (bp): 7842
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha ; DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pol
• Species: human spumaretrovirus (foamy virus)
• Insert Size (bp): 4152
• GenBank ID: U21247.1
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • Protocol for ΔΦ FV Vector Production

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ΔΦ vectors are “”Deleted Foamy” virus vectors that only retain essential cis-acting viral sequences. The 4 plasmids required to make ΔΦ vectors are pCiES, pCiGSΔΨ, pCiPS, and pΔΦ. The first three plasmids are helper constructs expressing Env, Gag, and Pol respectively. pΔΦ is a deleted foamy vector backbone with a polylinker for you to insert a transgene cassette. A description of the plasmids has been published (Trobridge et al., 2002).

For additional information, please see:
http://depts.washington.edu/dwrlab/reagents/foamy-virus-reagents/
http://depts.washington.edu/dwrlab/reagents/aav-serotype-reagents/