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Addgene

pMito-PAGFP-FRB.
(Plasmid #60005)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60005 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMito
  • Backbone size (bp) 5141
  • Modifications to backbone
    Insertion of PAGFP (C-terminal of MCS).
  • Vector type
    Mammalian Expression ; Knocksideways
  • Promoter CMV
  • Selectable markers
    Neomycin (select with G418)
  • Tags / Fusion Proteins
    • PAGFP (C terminal on backbone)
    • FRB (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Generated by modification of pMito-YFP-FRB plasmid - Gift from Scotte Robinson, Plasmid 46942.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMito-PAGFP-FRB. was a gift from Stephen Royle (Addgene plasmid # 60005 ; http://n2t.net/addgene:60005 ; RRID:Addgene_60005)

  • For your References section:

    Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension. Cheeseman LP, Harry EF, McAinsh AD, Prior IA, Royle SJ. J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. Epub 2013 Mar 26. 10.1242/jcs.124834 PubMed 23532825
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