pBrain-GFP-shGL2
(Plasmid #60004)

• Purpose: Co-expresses control GL2 shRNA and GFP.
• Depositing Lab: Stephen Royle
• Publication: Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 2011 Feb 4.

Backbone

• Vector backbone: pBrain
• Backbone manufacturer: Stephen J Royle
• Backbone size w/o insert (bp): 4733
• Total vector size (bp): 5447
• Modifications to backbone: Inclusion of control shRNA to target GL2 (firefly luciferase).
• Vector type: Mammalian Expression, RNAi
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EGFP
• gRNA/shRNA sequence: shGL2 - CGTACGCGGAATACTTCGA
• Species: A. victoria (Jellyfish), Photinus pyralis (Firefly)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ApaLI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry