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PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 59998 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneL4440
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Backbone manufacturerAndrew Fire
- Backbone size w/o insert (bp) 2790
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsUtilize HT115(DE3) cells for induction of dsRNA
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameklp-16
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Alt nameC41G7.2
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SpeciesC. elegans (nematode)
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Insert Size (bp)1170
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Mutationpartial genomic
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GenBank IDNM_060126
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Entrez Geneklp-16 (a.k.a. CELE_C41G7.2)
Gene/Insert 2
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Gene/Insert namehim-8
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Alt nameT07G12.12
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SpeciesC. elegans (nematode)
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Insert Size (bp)1021
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Mutationpartial genomic
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GenBank IDNM_069553
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Entrez Genehim-8 (a.k.a. CELE_T07G12.12)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Source BioScience LifeSciences C. elegans RNAi library clones utilized in plasmid construction (Fraser, A.G., Kamath, R.S., Zipperlen, P., Martinez-Campos, M., Sohrmann, M. and Ahringer, J. (2000) Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408, 325–330.)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLT 651 was a gift from Lisa Timmons (Addgene plasmid # 59998 ; http://n2t.net/addgene:59998 ; RRID:Addgene_59998) -
For your References section:
Systematic comparison of bacterial feeding strains for increased yield of Caenorhabditis elegans males by RNA interference-induced non-disjunction. Timmons L, Luna H, Martinez J, Moore Z, Nagarajan V, Kemege JM, Asad N. FEBS Lett. 2014 Jul 24. pii: S0014-5793(14)00572-9. doi: 10.1016/j.febslet.2014.07.023. 10.1016/j.febslet.2014.07.023 PubMed 25066299