pLT 653
(Plasmid #59996)

• Purpose: Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny
• Depositing Lab: Lisa Timmons
• Publication: Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(14)00572-9. doi: 10.1016/j.febslet.2014.07.023.

Backbone

• Vector backbone: L4440
• Backbone manufacturer: Andrew Fire
• Backbone size w/o insert (bp): 2790
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Utilize HT115(DE3) cells for induction of dsRNA
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: dhc-3
• Alt name: B0365.7
• Species: C. elegans (nematode)
• Insert Size (bp): 465
• Mutation: partial genomic
• GenBank ID: NM_073871
• Entrez Gene: dhc-3 (a.k.a. CELE_B0365.7)

Gene/Insert 2

• Gene/Insert name: klp-16
• Alt name: C41G7.2
• Species: C. elegans (nematode)
• Insert Size (bp): 1170
• Mutation: partial genomic
• GenBank ID: NM_060126
• Entrez Gene: klp-16 (a.k.a. CELE_C41G7.2)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Source BioScience LifeSciences C. elegans RNAi library clones utilized in plasmid construction (Fraser, A.G., Kamath, R.S., Zipperlen, P., Martinez-Campos, M., Sohrmann, M. and Ahringer, J. (2000) Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408, 325–330.)