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Addgene

Nanog-2A-mCherry
(Plasmid #59995)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59995 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PCR2_TOPO
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nanog
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Nanog (a.k.a. 2410002E02Rik, ENK, Stm1, ecat4)
  • Tag / Fusion Protein
    • 2A-mCherry (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Nanog-2A-mCherry was a gift from Rudolf Jaenisch (Addgene plasmid # 59995 ; http://n2t.net/addgene:59995 ; RRID:Addgene_59995)

  • For your References section:

    Single-cell analysis reveals that expression of nanog is biallelic and equally variable as that of other pluripotency factors in mouse ESCs. Faddah DA, Wang H, Cheng AW, Katz Y, Buganim Y, Jaenisch R. Cell Stem Cell. 2013 Jul 3;13(1):23-9. doi: 10.1016/j.stem.2013.04.019. 10.1016/j.stem.2013.04.019 PubMed 23827708
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