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PurposeInserts GFP immediately downstream of the Nanog coding region
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59994 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePCR2_TOPO
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Vector typeMouse Targeting
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNanog
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SpeciesM. musculus (mouse)
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Entrez GeneNanog (a.k.a. 2410002E02Rik, ENK, Stm1, ecat4)
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Tag
/ Fusion Protein
- 2A-GFP (C terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer lac_promoter
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Nanog-2A-GFP was a gift from Rudolf Jaenisch (Addgene plasmid # 59994 ; http://n2t.net/addgene:59994 ; RRID:Addgene_59994) -
For your References section:
Single-cell analysis reveals that expression of nanog is biallelic and equally variable as that of other pluripotency factors in mouse ESCs. Faddah DA, Wang H, Cheng AW, Katz Y, Buganim Y, Jaenisch R. Cell Stem Cell. 2013 Jul 3;13(1):23-9. doi: 10.1016/j.stem.2013.04.019. 10.1016/j.stem.2013.04.019 PubMed 23827708
