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Addgene

pSuper.Retro.Puro-PKN1 shRNA 4
(Plasmid #59978)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59978 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSuper.Retro.Puro
  • Backbone manufacturer
    OligoEngine
  • Backbone size w/o insert (bp) 6349
  • Total vector size (bp) 6709
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKN1 shRNA
  • gRNA/shRNA sequence
    CGAGAGAGATGCAGAAGAT
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer CGAACGCTGACGTCATC
  • 3′ sequencing primer GGAAGCCTTGGCTTTTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSuper.Retro.Puro-PKN1 shRNA 4 was a gift from Debu Chakravarti (Addgene plasmid # 59978 ; http://n2t.net/addgene:59978 ; RRID:Addgene_59978)

  • For your References section:

    A role for WDR5 in integrating threonine 11 phosphorylation to lysine 4 methylation on histone H3 during androgen signaling and in prostate cancer. Kim JY, Banerjee T, Vinckevicius A, Luo Q, Parker JB, Baker MR, Radhakrishnan I, Wei JJ, Barish GD, Chakravarti D. Mol Cell. 2014 May 22;54(4):613-25. doi: 10.1016/j.molcel.2014.03.043. Epub 2014 May 1. 10.1016/j.molcel.2014.03.043 PubMed 24793694
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