pEF1-puro/hpb1
(Plasmid
#59976)
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Purposeintegrin b1 short headpiece expression (beta-I, hybrid, PSI and I-EFG1 domains) expression
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59976 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF1-puro
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 7500
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman integrin b1 headpiece
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SpeciesH. sapiens (human)
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Insert Size (bp)1659
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Entrez GeneITGB1 (a.k.a. CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB)
- Promoter EF1a promoter
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Tag
/ Fusion Protein
- Base coiled-coil and His-tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site PmeI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer BGH_rev_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF1-puro/hpb1 was a gift from Timothy Springer (Addgene plasmid # 59976 ; http://n2t.net/addgene:59976 ; RRID:Addgene_59976)