pGEX-4T1-WDR5
(Plasmid
#59969)
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PurposeBacterial Expression GST-WDR5
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59969 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-4T1
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4900
- Total vector size (bp) 5905
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWDR5
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SpeciesH. sapiens (human)
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Insert Size (bp)1005
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GenBank IDNM_017588.2 NM_017588.2
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Entrez GeneWDR5 (a.k.a. BIG-3, BIG3, CFAP89, SWD3)
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-4T1-WDR5 was a gift from Debu Chakravarti (Addgene plasmid # 59969 ; http://n2t.net/addgene:59969 ; RRID:Addgene_59969) -
For your References section:
A role for WDR5 in integrating threonine 11 phosphorylation to lysine 4 methylation on histone H3 during androgen signaling and in prostate cancer. Kim JY, Banerjee T, Vinckevicius A, Luo Q, Parker JB, Baker MR, Radhakrishnan I, Wei JJ, Barish GD, Chakravarti D. Mol Cell. 2014 May 22;54(4):613-25. doi: 10.1016/j.molcel.2014.03.043. Epub 2014 May 1. 10.1016/j.molcel.2014.03.043 PubMed 24793694