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pOTTC446 - pAAV EF1a hPREP(S554A)
(Plasmid #59968)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59968 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOTTC374 - pAAV EF1a DIO iRFP
  • Backbone manufacturer
    Christopher Richie / OTTC / NIDA
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Prolyl oligopeptidase
  • Alt name
    PREP
  • Species
    H. sapiens (human)
  • Mutation
    S554A
  • GenBank ID
    NP_002717.3
  • Entrez Gene
    PREP (a.k.a. PE, PEP)
  • Promoter EF1a

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer EF1a_F1
  • 3′ sequencing primer WPRE_R1
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC446 - pAAV EF1a hPREP(S554A) was a gift from Brandon Harvey (Addgene plasmid # 59968 ; http://n2t.net/addgene:59968 ; RRID:Addgene_59968)

  • For your References section:

    The beneficial effect of a prolyl oligopeptidase inhibitor, KYP-2047, on alpha-synuclein clearance and autophagy in A30P transgenic mouse. Savolainen MH, Richie CT, Harvey BK, Mannisto PT, Maguire-Zeiss KA, Myohanen TT. Neurobiol Dis. 2014 Aug;68:1-15. doi: 10.1016/j.nbd.2014.04.003. Epub 2014 Apr 16. 10.1016/j.nbd.2014.04.003 PubMed 24746855
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