pTHSSd_1
(Plasmid
#59947)
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PurposeSplitposon in KanR ColE1 backbone
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59947 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSB1C3
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSplitposon w/ KanR, LacI & PTac
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SpeciesSynthetic
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MutationN/A
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Other
- 3′ sequencing primer Other (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTHSSd_1 was a gift from Christopher Voigt (Addgene plasmid # 59947 ; http://n2t.net/addgene:59947 ; RRID:Addgene_59947) -
For your References section:
A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA. Mol Syst Biol. 2014 Jul 30;10(7):742. 10.15252/msb.20145299 PubMed 25080493
Map uploaded by the depositor.
