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pJK_proB_amiE
(Plasmid #59836)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59836 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-29b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3582
  • Total vector size (bp) 4605
  • Modifications to backbone
    Deletion of lacI, lacO, and T7 promoter by cloning proB in-between BglI and XbaI. Removal of the kanamycin resistance gene and insertion of the beta-lactamase (AmpR) gene from pET-22b.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ALIPHATIC AMIDASE
  • Alt name
    amiE
  • Insert Size (bp)
    1024
  • Entrez Gene
    amiE (a.k.a. PA3366)
  • Promoter proB
  • Tag / Fusion Protein
    • 6x-His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GGTGATTCATTCTGCTAA
  • 3′ sequencing primer T7 Term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK_proB_amiE was a gift from Timothy Whitehead (Addgene plasmid # 59836 ; http://n2t.net/addgene:59836 ; RRID:Addgene_59836)

  • For your References section:

    The Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead TA. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. 10.1371/journal.pone.0109105 PubMed 25286161
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