pcDNA5 FRT TO myc p31Comet
(Plasmid
#59833)
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PurposeAllows the integration of myc p31Comet in the genome and Tet-inducible expression.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59833 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5/FRT/TO
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep31
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SpeciesH. sapiens (human)
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Entrez GeneMAD2L1BP (a.k.a. CMT2)
- Promoter hybrid human cytomegalovirus (CMV)/TetO2 promoter
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5 FRT TO myc p31Comet was a gift from Stephen Taylor (Addgene plasmid # 59833 ; http://n2t.net/addgene:59833 ; RRID:Addgene_59833) -
For your References section:
p31comet-mediated extraction of Mad2 from the MCC promotes efficient mitotic exit. Westhorpe FG, Tighe A, Lara-Gonzalez P, Taylor SS. J Cell Sci. 2011 Nov 15;124(Pt 22):3905-16. doi: 10.1242/jcs.093286. Epub 2011 Nov 18. 10.1242/jcs.093286 PubMed 22100920
