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Addgene

pcDNA5 FRT TO myc Separase delta 1278-1291
(Plasmid #59830)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59830 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Separase
  • Species
    H. sapiens (human)
  • Mutation
    delta 1278-1291
  • Entrez Gene
    ESPL1 (a.k.a. ESP1, SEPA)
  • Promoter hybrid human cytomegalovirus (CMV)/TetO2 promoter
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5 FRT TO myc Separase delta 1278-1291 was a gift from Stephen Taylor (Addgene plasmid # 59830 ; http://n2t.net/addgene:59830 ; RRID:Addgene_59830)

  • For your References section:

    Protein phosphatase 2A and separase form a complex regulated by separase autocleavage. Holland AJ, Bottger F, Stemmann O, Taylor SS. J Biol Chem. 2007 Aug 24;282(34):24623-32. Epub 2007 Jun 29. 10.1074/jbc.M702545200 PubMed 17604273
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