pcDNA5 FRT TO myc Separase delta 55/S1126A
(Plasmid
#59825)
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PurposeAllows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59825 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5/FRT/TO
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSeparase
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SpeciesH. sapiens (human)
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Mutationdelta 55/S1126A
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Entrez GeneESPL1 (a.k.a. ESP1, SEPA)
- Promoter hybrid human cytomegalovirus (CMV)/TetO2 promoter
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5 FRT TO myc Separase delta 55/S1126A was a gift from Stephen Taylor (Addgene plasmid # 59825 ; http://n2t.net/addgene:59825 ; RRID:Addgene_59825) -
For your References section:
Protein phosphatase 2A and separase form a complex regulated by separase autocleavage. Holland AJ, Bottger F, Stemmann O, Taylor SS. J Biol Chem. 2007 Aug 24;282(34):24623-32. Epub 2007 Jun 29. 10.1074/jbc.M702545200 PubMed 17604273
