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Addgene

pcDNA5 FRT TO myc Separase S1226A/ C2029A
(Plasmid #59823)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59823 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Separase
  • Species
    H. sapiens (human)
  • Mutation
    S1226A/ C2029A
  • Entrez Gene
    ESPL1 (a.k.a. ESP1, SEPA)
  • Promoter hybrid human cytomegalovirus (CMV)/TetO2 promoter
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5 FRT TO myc Separase S1226A/ C2029A was a gift from Stephen Taylor (Addgene plasmid # 59823 ; http://n2t.net/addgene:59823 ; RRID:Addgene_59823)

  • For your References section:

    Cyclin-B1-mediated inhibition of excess separase is required for timely chromosome disjunction. Holland AJ, Taylor SS. J Cell Sci. 2006 Aug 15;119(Pt 16):3325-36. Epub 2006 Jul 25. 10.1242/jcs.03083 PubMed 16868023
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