pcDNA5 FRT TO GFP hBub1 K821R
(Plasmid
#59813)
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PurposeAllows the integration of GFP hBub1 K821R in the genome and Tet-inducible expression.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5/FRT/TO
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBub1
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SpeciesH. sapiens (human)
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MutationK821R
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Entrez GeneBUB1 (a.k.a. BUB1A, BUB1L, hBUB1)
- Promoter hybrid human cytomegalovirus (CMV)/TetO2 promoter
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Tag
/ Fusion Protein
- EmGFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5 FRT TO GFP hBub1 K821R was a gift from Stephen Taylor (Addgene plasmid # 59813 ; http://n2t.net/addgene:59813 ; RRID:Addgene_59813) -
For your References section:
Sgo1 establishes the centromeric cohesion protection mechanism in G2 before subsequent Bub1-dependent recruitment in mitosis. Perera D, Taylor SS. J Cell Sci. 2010 Mar 1;123(Pt 5):653-9. doi: 10.1242/jcs.059501. Epub 2010 Feb 2. 10.1242/jcs.059501 PubMed 20124418
