pcDNA5 FRT TO Myc ArC K72R
(Plasmid #59811)

• Purpose: Allows the integration of Myc ArC K72R in the genome and Tet-inducible expression.
• Depositing Lab: Stephen Taylor
• Publication: Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-75. Epub 2006 Aug 15.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Aurora C
• Species: H. sapiens (human)
• Mutation: K72R, kinase mutant
• Entrez Gene: AURKC (a.k.a. AIE2, AIK3, ARK3, AurC, HEL-S-90, SPGF5, STK13, aurora-C)
• Promoter: hybrid human cytomegalovirus (CMV)/TetO2 promoter
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry