pcDNA5 FRT TO Myc ArA K162R
(Plasmid #59805)

• Purpose: Allows the integration of Myc ArA K162R in the genome and Tet-inducible expression
• Depositing Lab: Stephen Taylor
• Publication: Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-75. Epub 2006 Aug 15.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Aurora A
• Species: H. sapiens (human)
• Mutation: K162R, kinase mutant
• Entrez Gene: AURKA (a.k.a. AIK, ARK1, AURA, BTAK, PPP1R47, STK15, STK6, STK7)
• Promoter: hybrid human cytomegalovirus (CMV)/TetO2 promoter
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry