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pSA120
(Plasmid #59789)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59789 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFJ150
  • Modifications to backbone
    modified to include PmeI restriction digest site for Gibson cloning
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    hsp-16.41 promoter
  • Species
    C. elegans (nematode)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gatcaccaaaaacggaacgttgagc
  • 3′ sequencing primer TTTCGAAGTTTTTTAGATGCACTAGAACAAAGCGTG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    zif-1 coding
  • Species
    C. elegans (nematode)

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATGAGTGAGTGTTCCGCGAGTA
  • 3′ sequencing primer TTATTGTTGAATATTTATCGTGCACAAATTTTCGGCAG
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    gpd-2/gpd-3 intergenic sequence
  • Species
    C. elegans (nematode)

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gctgtctcatcctactttcacctag
  • 3′ sequencing primer GATGCGTTGAAGCAGTTTCCCTG
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    mCherry (worm)
  • Species
    C. elegans (nematode)

Cloning Information for Gene/Insert 4

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATGGTCTCAAAGGGTGAAGAAG
  • 3′ sequencing primer CTACTTATACAATTCATCCATGCCAC
    • (Common Sequencing Primers)

Gene/Insert 5

  • Gene/Insert name
    unc-54 3' UTR
  • Species
    C. elegans (nematode)

Cloning Information for Gene/Insert 5

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gtccaattactcttcaacatccc
  • 3′ sequencing primer AAACAGTTATGTTTGGTATATTGGGAATG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSA120 was a gift from Jeremy Nance (Addgene plasmid # 59789 ; http://n2t.net/addgene:59789 ; RRID:Addgene_59789)

  • For your References section:

    Repurposing an endogenous degradation system for rapid and targeted depletion of C. elegans proteins. Armenti ST, Lohmer LL, Sherwood DR, Nance J. Development. 2014 Dec;141(23):4640-7. doi: 10.1242/dev.115048. Epub 2014 Nov 5. 10.1242/dev.115048 PubMed 25377555
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