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Addgene

pSA101
(Plasmid #59787)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59787 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFJ150
  • Modifications to backbone
    modified to include PmeI restriction site for Gibson cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    rab-3 promoter
  • Species
    C. elegans (nematode)
  • GenBank ID

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer atcttcagatgggagcagtggactgtc
  • 3′ sequencing primer CTGAAAATAGGGCTACTGTAGATTTATTTTAAAAGAG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    zif-1 coding
  • Species
    C. elegans (nematode)
  • Entrez Gene
    zif-1 (a.k.a. CELE_F59B2.6)

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer atgagtgagtgttccgcgagtaccagtcagttatc
  • 3′ sequencing primer TTATTGTTGAATATTTATCGTGCACAAATTTTCGGCAG
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    unc-54 3' UTR
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    705
  • GenBank ID

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gtccaattactcttcaacatccctacatgctctttctccc
  • 3′ sequencing primer AAACAGTTATGTTTGGTATATTGGGAATGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSA101 was a gift from Jeremy Nance (Addgene plasmid # 59787 ; http://n2t.net/addgene:59787 ; RRID:Addgene_59787)

  • For your References section:

    Repurposing an endogenous degradation system for rapid and targeted depletion of C. elegans proteins. Armenti ST, Lohmer LL, Sherwood DR, Nance J. Development. 2014 Dec;141(23):4640-7. doi: 10.1242/dev.115048. Epub 2014 Nov 5. 10.1242/dev.115048 PubMed 25377555
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