pSA109
(Plasmid #59783)

• Purpose: drives ZIF-1 protein in C. elegans intestinal cells
• Depositing Lab: Jeremy Nance
• Publication: Armenti et al Development. 2014 Dec;141(23):4640-7. doi: 10.1242/dev.115048. Epub 2014 Nov 5.

Backbone

• Vector backbone: pCFJ150
• Modifications to backbone: vector modified to include PmeI restriction site for Gibson cloning

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: elt-2 promoter
• Species: C. elegans (nematode)
• Insert Size (bp): 5002
• Entrez Gene: elt-2 (a.k.a. CELE_C33D3.1)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ggtcgagctgaatacacgtgc
• 3′ sequencing primer: CTATAATCTATTTTCTAGTTTCTATTTTATTAGAATGC

Gene/Insert 2

• Gene/Insert name: zif-1 coding sequence
• Species: C. elegans (nematode)
• Insert Size (bp): 1746
• Entrez Gene: zif-1 (a.k.a. CELE_F59B2.6)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: atgagtgagtgttccgcgagtac
• 3′ sequencing primer: TTATTGTTGAATATTTATCGTGCACAAATTTTCGGCAG

Gene/Insert 3

• Gene/Insert name: unc-54 3' UTR
• Species: C. elegans (nematode)

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gtccaattactcttcaacatccc
• 3′ sequencing primer: AAACAGTTATGTTTGGTATATTGGGAATG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry