pCMV-OptoFGFR1-Y766F
(Plasmid #59777)

• Purpose: Expresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1
• Depositing Lab: Won Do Heo
• Publication: Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi: 10.1016/j.chembiol.2014.05.013. Epub 2014 Jun 26.

Backbone

• Vector backbone: pmCitrine-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4733
• Total vector size (bp): 7550
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OptoFGFR1-Y766F
• Alt name: Lyn-cytoFGFR1(Y766F)-PHR-mCit
• Species: H. sapiens (human), A. thaliana (mustard weed)
• Insert Size (bp): 3617
• GenBank ID: NM_023110.2
• Entrez Gene: FGFR1 (a.k.a. BFGFR, CD331, CEK, ECCL, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCitrine (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40-pAR

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry