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GFP-APPL1-R146A/K152A/R154A
(Plasmid #59767)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59767 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2
  • Total vector size (bp) 7400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    APPL1
  • Species
    H. sapiens (human)
  • Mutation
    R146A/K152A/R154A
  • GenBank ID
    NM_012096.2
  • Entrez Gene
    APPL1 (a.k.a. APPL, DIP13alpha, MODY14)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer TCGAATTCGGCTTATGCCGG
  • 3′ sequencing primer TCGGTACCATGCTTCTGATTCTCTCTTCTTTCCTCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-APPL1-R146A/K152A/R154A was a gift from Donna Webb (Addgene plasmid # 59767 ; http://n2t.net/addgene:59767 ; RRID:Addgene_59767)

  • For your References section:

    The endosomal adaptor protein APPL1 impairs the turnover of leading edge adhesions to regulate cell migration. Broussard JA, Lin WH, Majumdar D, Anderson B, Eason B, Brown CM, Webb DJ. Mol Biol Cell. 2012 Apr;23(8):1486-99. doi: 10.1091/mbc.E11-02-0124. Epub 2012 Feb 29. 10.1091/mbc.E11-02-0124 PubMed 22379109
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