Skip to main content
Addgene

pBSWt1-Dest
(Plasmid #59764)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59764 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBluescript SKII-
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    attL1-em7-kanR-attL2-pA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GTGCGTCCAGCAGCCGGAGCAA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSWt1-Dest was a gift from Peter Koopman (Addgene plasmid # 59764 ; http://n2t.net/addgene:59764 ; RRID:Addgene_59764)

  • For your References section:

    A piggyBac transposon- and gateway-enhanced system for efficient BAC transgenesis. Zhao L, Ng ET, Koopman P. Dev Dyn. 2014 Jun 12. doi: 10.1002/dvdy.24153. 10.1002/dvdy.24153 PubMed 24924516
Related items: