tol2-CAG::ChR2-YFP
(Plasmid #59740)

• Purpose: Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)
• Depositing Lab: Ivo Lieberam
• Publication: Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi: 10.1126/science.1248523.

Backbone

• Vector backbone: pUC18
• Modifications to backbone: tol2L/R flanked expression vector consisting of CAG promoter, followed by a 5' splice substrate, the ChR2-YFP gene, a bGH poly-adenylation sequence, and a FRT-zeo-FRT resistance marker.
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Channelrhodopsin-2
• Alt name: ChR2
• Species: Chlamydomonas reinhardtii
• Mutation: H134R
• Promoter: CAG
• Tag / Fusion Protein:
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCAGGS5
• 3′ sequencing primer: Zeo-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry