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Addgene

pET14b_SG
(Plasmid #59737)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59737 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET14b
  • Backbone manufacturer
    EMD Millipore
  • Backbone size w/o insert (bp) 4671
  • Total vector size (bp) 5553
  • Modifications to backbone
    Thrombin cleavage site of pET14b replaced by TEV protease cleavage site.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    zonula occludens-1
  • Alt name
    TJP-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    882
  • Entrez Gene
    TJP1 (a.k.a. ZO-1)
  • Tags / Fusion Proteins
    • HIS tag (N terminal on backbone)
    • thrombin clvg (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (not destroyed)
  • 3′ cloning site Bam HI (not destroyed)
  • 5′ sequencing primer T7 fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET14b_SG was a gift from Alan Fanning (Addgene plasmid # 59737 ; http://n2t.net/addgene:59737 ; RRID:Addgene_59737)

  • For your References section:

    Insights into regulated ligand binding sites from the structure of ZO-1 Src homology 3-guanylate kinase module. Lye MF, Fanning AS, Su Y, Anderson JM, Lavie A. J Biol Chem. 2010 Apr 30;285(18):13907-17. doi: 10.1074/jbc.M109.093674. Epub 2010 Mar 3. 10.1074/jbc.M109.093674 PubMed 20200156
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