pGEX-2T_PDZ2
(Plasmid
#59735)
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Purposeexpresses PDZ2 domain of human ZO-1 in bacteria
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59735 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-2T
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Backbone manufacturerGE Healthcare Life Sciences
- Backbone size w/o insert (bp) 4948
- Total vector size (bp) 5196
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Modifications to backbonenone
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namezonula occludens-1
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Alt nameTJP-1
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SpeciesH. sapiens (human)
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Insert Size (bp)248
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Entrez GeneTJP1 (a.k.a. ZO-1)
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- thrombin clvg (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Eco RI (not destroyed)
- 5′ sequencing primer pGEX 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-2T_PDZ2 was a gift from Alan Fanning (Addgene plasmid # 59735 ; http://n2t.net/addgene:59735 ; RRID:Addgene_59735) -
For your References section:
Domain swapping within PDZ2 is responsible for dimerization of ZO proteins. Fanning AS, Lye MF, Anderson JM, Lavie A. J Biol Chem. 2007 Dec 28;282(52):37710-6. Epub 2007 Oct 9. 10.1074/jbc.M707255200 PubMed 17928286