MC177VSG221RNAi Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid
MC177VSG221RNAi with the following enzymes: HindIII HF, XhoI and HindIII HF + XhoI
We expect the linearized plasmid to run at ~6kb and the double digest to give bands of ~0.8kb and 5.2 kb
Our results indicate that the linear plasmid is the correct size and the HindIII site was destroyed during cloning.
