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Addgene

Nanog-CreER targeting construct
(Plasmid #59720)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59720 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDT
  • Total vector size (bp) 15024
  • Vector type
    Mouse Targeting
  • Selectable markers
    Hygromycin ; DT

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    creERT2
  • Species
    Synthetic
  • Promoter mNanog

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The backbone contains DT gene for negative selection.

Please visit https://www.biorxiv.org/content/early/2014/08/23/008284 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Nanog-CreER targeting construct was a gift from Jacob Hanna (Addgene plasmid # 59720 ; http://n2t.net/addgene:59720 ; RRID:Addgene_59720)

  • For your References section:

    Transient acquisition of pluripotency during somatic cell transdifferentiation with iPSC reprogramming factors. Maza I, Caspi I, Zviran A, Chomsky E, Rais Y, Viukov S, Geula S, Buenrostro JD, Weinberger L, Krupalnik V, Hanna S, Zerbib M, Dutton JR, Greenleaf WJ, Massarwa R, Novershtern N, Hanna JH. Nat Biotechnol. 2015 Jun 22. doi: 10.1038/nbt.3270. 10.1038/nbt.3270 PubMed 26098448
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