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Addgene

pSicoR-SLC17A5i1
(Plasmid #59598)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59598 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSicoR
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLC17A5
  • gRNA/shRNA sequence
    SLC17A5
  • Species
    H. sapiens (human)
  • Entrez Gene
    SLC17A5 (a.k.a. AST, ISSD, NSD, SD, SIALIN, SIASD, SLD)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer mU6-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSicoR-SLC17A5i1 was a gift from Miguel Ramalho-Santos (Addgene plasmid # 59598 ; http://n2t.net/addgene:59598 ; RRID:Addgene_59598)

  • For your References section:

    Systematic Identification of Barriers to Human iPSC Generation. Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust EM, McManus MT, Song JS, Ramalho-Santos M. Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040. 10.1016/j.cell.2014.05.040 PubMed 25036638
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