Skip to main content
Addgene

pSicoR-UBE2D3i1
(Plasmid #59586)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59586 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSicoR
  • Vector type
    Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UBE2D3
  • gRNA/shRNA sequence
    UBE2D3
  • Species
    H. sapiens (human)
  • Entrez Gene
    UBE2D3 (a.k.a. E2(17)KB3, UBC4/5, UBCH5C)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer mU6-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSicoR-UBE2D3i1 was a gift from Miguel Ramalho-Santos (Addgene plasmid # 59586 ; http://n2t.net/addgene:59586 ; RRID:Addgene_59586)

  • For your References section:

    Systematic Identification of Barriers to Human iPSC Generation. Qin H, Diaz A, Blouin L, Lebbink RJ, Patena W, Tanbun P, LeProust EM, McManus MT, Song JS, Ramalho-Santos M. Cell. 2014 Jul 17;158(2):449-61. doi: 10.1016/j.cell.2014.05.040. 10.1016/j.cell.2014.05.040 PubMed 25036638
Related items:
Commonly requested with: