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Addgene

ReaLiSM A1-26link-GPIba+2cys
(Plasmid #59580)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59580 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEXM
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 6100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VWF A1-linker-GPIba
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Entrez Gene
    VWF (a.k.a. F8VWF, VWD)
  • Promoter chick beta-actin
  • Tag / Fusion Protein
    • 6His (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ATGGATATGAGAGTT
  • 3′ sequencing primer TTACGCGTGATGGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ReaLiSM A1-26link-GPIba+2cys was a gift from Timothy Springer (Addgene plasmid # 59580 ; http://n2t.net/addgene:59580 ; RRID:Addgene_59580)

  • For your References section:

    A mechanically stabilized receptor-ligand flex-bond important in the vasculature. Kim J, Zhang CZ, Zhang X, Springer TA. Nature. 2010 Aug 19;466(7309):992-5. doi: 10.1038/nature09295. 10.1038/nature09295 PubMed 20725043
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