ReaLiSM A1-43-GPIba+2cys
(Plasmid
#59577)
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Purposeexpress VWF A1-GPIba ReaLiSM construct for single molecule study
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 59577 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLEXM
- Backbone size w/o insert (bp) 4500
- Total vector size (bp) 6200
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVWF A1-linker-GPIba
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SpeciesH. sapiens (human)
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Insert Size (bp)1700
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Entrez GeneVWF (a.k.a. F8VWF, VWD)
- Promoter chick beta-actin
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Tag
/ Fusion Protein
- 6His (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer ATGGATATGAGAGTT
- 3′ sequencing primer TTACGCGTGATGGTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ReaLiSM A1-43-GPIba+2cys was a gift from Timothy Springer (Addgene plasmid # 59577 ; http://n2t.net/addgene:59577 ; RRID:Addgene_59577)