pREMI-Z
(Plasmid
#59527)
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Purpose(Empty Backbone) Zeocin-resistance plasmid for insertional mutagenesis.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepREMI-Z
- Backbone size (bp) 1986
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Vector typeYeast Expression
- Promoter TEF1
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13rev
- 3′ sequencing primer CYC1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Linearize at BamHI.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pREMI-Z was a gift from Benjamin Glick (Addgene plasmid # 59527 ; http://n2t.net/addgene:59527 ; RRID:Addgene_59527) -
For your References section:
Paz2 and 13 other PAZ gene products regulate vacuolar engulfment of peroxisomes during micropexophagy. Mukaiyama H, Oku M, Baba M, Samizo T, Hammond AT, Glick BS, Kato N, Sakai Y. Genes Cells. 2002 Jan;7(1):75-90. 499 [pii] PubMed 11856375
Map uploaded by the depositor.
