RP185 pUAST EGFP:Or47b
(Plasmid #59525)

• Purpose: N-terminal fusion of GFP to Drosophila melanogaster Or47b
• Depositing Lab: Leslie Vosshall
• Publication: Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006 Jan 17.

Backbone

• Vector backbone: pUAST
• Backbone size w/o insert (bp): 8904
• Total vector size (bp): 10604
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EGFP:Or47b
• Alt name: Or47b
• Alt name: Odorant receptor 47b
• Species: D. melanogaster (fly)
• Insert Size (bp): 2100
• Entrez Gene: Or47b (a.k.a. Dmel_CG13206, 47E.2, 47b, CG13206, DOR25, DOR47E.2, Dmel\CG13206, OR 47b, OR47b, Or47E.2)
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: Pry1 (5'-ccttagcatgtccgtggggtttgaat-3'); EGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3')
• 3′ sequencing primer: SV40-splice-R2 (5'-CACAAACAATTAGAATCAGTAG-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Compared to the NCBI genomic reference sequence for Drosophila melanogaster Or47b , Addgene's quality control sequencing finds about ten mismatches in the insert ORF, including I245M and G363D amino acid residue substitutions. The depositing laboratory is aware of these differences, and states that they are polymorphisms that are not expected to affect protein function. The coding changes are not in highly conserved domains in the protein.