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RP132 pUAST EGFP:Or43a
(Plasmid #59523)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59523 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 8904
  • Total vector size (bp) 11104
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFP:Or43a
  • Alt name
    Or43a
  • Alt name
    Odorant receptor 43a
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2200
  • Entrez Gene
    Or43a (a.k.a. Dmel_CG1854, 43B.1, 43B1, 43a, AN14, CG1854, DOR43B.1, DOR43a, DOR87, Dmel\CG1854, Dor87, OR43a, Or, Or43A, Or43B.1, Or87, dor87, or43a)
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer Pry1 (5'-ccttagcatgtccgtggggtttgaat-3'); EGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3')
  • 3′ sequencing primer SV40-splice-R2 (5'-CACAAACAATTAGAATCAGTAG-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RP132 pUAST EGFP:Or43a was a gift from Leslie Vosshall (Addgene plasmid # 59523 ; http://n2t.net/addgene:59523 ; RRID:Addgene_59523)

  • For your References section:

    Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. Benton R, Sachse S, Michnick SW, Vosshall LB. PLoS Biol. 2006 Feb;4(2):e20. Epub 2006 Jan 17. 10.1371/journal.pbio.0040020 PubMed 16402857
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