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Addgene

pHRIG-S6K
(Plasmid #59446)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59446 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR-IG (pHR’tripCMV-IRES-eGFP)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    S6K
  • Alt name
    Rps6kb1
  • Species
    R. norvegicus (rat)
  • Mutation
    S6K starts at aa24 and contains 7 extra amino acids at end of S6K
  • Entrez Gene
    Rps6kb1 (a.k.a. p70 S6K-alpha)
  • Promoter CMV
  • Tag / Fusion Protein
    • IRES-EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pCDH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The S6K1 gene was cloned from Addgene plasmid # 26610, pcDNA-Myc S6K1. It starts at aa24 compared to GenBank reference sequence NP_114191.1 and contains an additional 7 amino acids after the end of S6K1 gene in this reference sequence and plasmid 26610.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHRIG-S6K was a gift from Heng Zhao (Addgene plasmid # 59446 ; http://n2t.net/addgene:59446 ; RRID:Addgene_59446)

  • For your References section:

    Mammalian Target of Rapamycin Cell Signaling Pathway Contributes to the Protective Effects of Ischemic Postconditioning Against Stroke. Xie R, Wang P, Cheng M, Sapolsky R, Ji X, Zhao H. Stroke. 2014 Jul 10. pii: STROKEAHA.114.005406. 10.1161/STROKEAHA.114.005406 PubMed 25013017
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