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RNAseq_cal_Tuschl
(Plasmid #59426)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59426 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMA-RQ-Bb
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 2341
  • Total vector size (bp) 3549

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RNAseq_cal_Tuschl
  • Insert Size (bp)
    1208

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site SfiI (not destroyed)
  • 5′ sequencing primer Custom made
  • 3′ sequencing primer Custom made
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Custom gene synthesis from Life Technologies

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See also PMID: 34850840 for an additional reference.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RNAseq_cal_Tuschl was a gift from Thomas Tuschl (Addgene plasmid # 59426 ; http://n2t.net/addgene:59426 ; RRID:Addgene_59426)

  • For your References section:

    Human plasma and serum extracellular small RNA reference profiles and their clinical utility. Max KEA, Bertram K, Akat KM, Bogardus KA, Li J, Morozov P, Ben-Dov IZ, Li X, Weiss ZR, Azizian A, Sopeyin A, Diacovo TG, Adamidi C, Williams Z, Tuschl T. Proc Natl Acad Sci U S A. 2018 Jun 5;115(23):E5334-E5343. doi: 10.1073/pnas.1714397115. Epub 2018 May 18. 10.1073/pnas.1714397115 PubMed 29777089
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