pCAG-HA-Nrxn1beta AS4(+)
(Plasmid #59410)

• Purpose: Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]
• Depositing Lab: Peter Scheiffele
• Publication: Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Backbone

• Vector backbone: pCAG
• Backbone size w/o insert (bp): 4867
• Total vector size (bp): 6310
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nrxn1beta AS4(+)
• Alt name: Neurexin 1beta AS4(+)
• Alt name: Neurexin 1beta Splice4
• Species: M. musculus (mouse)
• Insert Size (bp): 1443
• Entrez Gene: Nrxn1 (a.k.a. 1700062G21Rik, 9330127H16Rik, A230068P09Rik, mKIAA0578)
• Promoter: CAG
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
• 3′ sequencing primer: Bglob-pA-R (5'-TTTTGGCAGAGGGAAAAAGA-3'); pCAG_rev_seq (5'-CAGCCACCACCTTCTGATAG-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI).
The Nrxn 1 beta variant encoded by this plasmid is AS4(+) / AS5(+):
- Exon 21 constituting alternatively spliced segment 4 (AS4) (=ENSMUSE00001309616) is present.
- AS5 is present, i.e. the long form of exon 23 (23.1) is present (=ENSMUSE00000862714; "GTGGGTTAG" in addition to shorter) exon 23.2 (=ENSMUSE00001290978)).
See Schreiner et al., Neuron, 2014 for nomenclature of alternatively spliced segments (doi:10.1016/j.neuron.2014.09.011).