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Addgene

pCAG-HA-Nrxn1beta AS4(+)
(Plasmid #59410)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59410 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCAG
  • Backbone size w/o insert (bp) 4867
  • Total vector size (bp) 6310
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nrxn1beta AS4(+)
  • Alt name
    Neurexin 1beta AS4(+)
  • Alt name
    Neurexin 1beta Splice4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1443
  • Entrez Gene
    Nrxn1 (a.k.a. 1700062G21Rik, 9330127H16Rik, A230068P09Rik, mKIAA0578)
  • Promoter CAG
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer Bglob-pA-R (5'-TTTTGGCAGAGGGAAAAAGA-3'); pCAG_rev_seq (5'-CAGCCACCACCTTCTGATAG-3')
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI).
The Nrxn 1 beta variant encoded by this plasmid is AS4(+) / AS5(+):
- Exon 21 constituting alternatively spliced segment 4 (AS4) (=ENSMUSE00001309616) is present.
- AS5 is present, i.e. the long form of exon 23 (23.1) is present (=ENSMUSE00000862714; "GTGGGTTAG" in addition to shorter) exon 23.2 (=ENSMUSE00001290978)).
See Schreiner et al., Neuron, 2014 for nomenclature of alternatively spliced segments (doi:10.1016/j.neuron.2014.09.011).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-HA-Nrxn1beta AS4(+) was a gift from Peter Scheiffele (Addgene plasmid # 59410 ; http://n2t.net/addgene:59410 ; RRID:Addgene_59410)
  • For your References section:

    Alternative splicing controls selective trans-synaptic interactions of the neuroligin-neurexin complex. Chih B, Gollan L, Scheiffele P. Neuron. 2006 Jul 20. 51(2):171-8. 10.1016/j.neuron.2006.06.005 PubMed 16846852