pTE104
(Plasmid
#59399)
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Purpose(Empty Backbone) MethyloBrick expression vector, coxB promoter; TcR
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59399 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTE100
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Backbone manufacturerErb Lab
- Backbone size (bp) 6558
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Modifications to backboneintroduced coxB promoter region
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Vector typeBacterial Expression, Synthetic Biology
- Promoter PcoxB
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Brick_fw_Seq
- 3′ sequencing primer pCM80_rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTE104 was a gift from Tobias Erb (Addgene plasmid # 59399 ; http://n2t.net/addgene:59399 ; RRID:Addgene_59399) -
For your References section:
A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. Schada von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt JA, Erb TJ. ACS Synth Biol. 2014 Aug 8. 10.1021/sb500221v PubMed 25105793
Map uploaded by the depositor.
