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Addgene

pTE101
(Plasmid #59396)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59396 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLM01
  • Backbone manufacturer
    Vorholt Lab
  • Backbone size (bp) 4841
  • Modifications to backbone
    removed promoter region; introduced new MCS
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Promoter none

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The original backbone plasmid, pLM01, is published in Kaczmarczyk et al., 2011, Journal of Bacteriology: Role of Sphingomonas sp. strain Fr1 PhyR-NepR-σEcfG cascade in general stress response and identification of a negative regulator of PhyR

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTE101 was a gift from Tobias Erb (Addgene plasmid # 59396 ; http://n2t.net/addgene:59396 ; RRID:Addgene_59396)

  • For your References section:

    A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. Schada von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt JA, Erb TJ. ACS Synth Biol. 2014 Aug 8. 10.1021/sb500221v PubMed 25105793
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