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Addgene

pTE100
(Plasmid #59395)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59395 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCM80
  • Backbone manufacturer
    Lidstrom Lab
  • Backbone size (bp) 7245
  • Modifications to backbone
    removed promoter region; introduced new MCS
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Promoter none

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The original backbone plasmid, pCM80, is published in Marx & Lidstrom, 2001, Microbiology: Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTE100 was a gift from Tobias Erb (Addgene plasmid # 59395 ; http://n2t.net/addgene:59395 ; RRID:Addgene_59395)

  • For your References section:

    A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. Schada von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt JA, Erb TJ. ACS Synth Biol. 2014 Aug 8. 10.1021/sb500221v PubMed 25105793
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